› 7tsw

X-ray diffraction
2.4Å resolution

Crystal Structure Of Human NADH-Cytochrome B5 Reductase T117D Mutant

Released:
DOI: 10.2210/pdb7tsw/pdb
PDB entry 7tsw coloured by chain, front view.
PDB entry 7tsw coloured by chain, side view.
PDB entry 7tsw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zheng A, Thibodeau PH

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 274 amino acids
Theoretical weight: 31.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00387 (Residues: 28-301; Coverage: 91%)
Gene names: CYB5R3, DIA1
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 6 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 162.562Å b: 79.986Å c: 172.358Å
α: 90° β: 98.672° γ: 90°
R-values:
R R work R free
0.228 0.227 0.264
Expression system: Escherichia coli

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