› 7tt3

Electron Microscopy
4.3Å resolution

BamABCDE bound to substrate EspP class 5

Released:
DOI: 10.2210/pdb7tt3/pdb
PDB entry 7tt3 coloured by chain, front view.
PDB entry 7tt3 coloured by chain, side view.
PDB entry 7tt3 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle MT, Jimah JR, Dowdy T, Ohlemacher SI, Larion M, Hinshaw JE, Bernstein HD
Cell 185 1143-1156.e13 (2022)
PMID: 35294859
Related structures: EMD-26109

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-128463 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 89.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 22-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 226 amino acids
Theoretical weight: 25.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC02 (Residues: 20-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamC Chain: C
Outer membrane protein assembly factor BamE Chain: E
Outer membrane protein assembly factor BamB Chain: B
Autotransporter protein EspP translocator; Maltose/maltodextrin-binding periplasmic protein Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.3Å
Relevant EMDB volumes: EMD-26109
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Surveying membrane landscapes: a new look at the bacterial cell surface.
Lithgow et al. (2023)
6 more

2 mentions without citation

Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle et al. (2022)
1 more