› 7u05

Electron Microscopy
3.7Å resolution

Structure of the yeast TRAPPII-Rab11/Ypt32 complex in the closed/closed state (composite structure)

Released:
DOI: 10.2210/pdb7u05/pdb
PDB entry 7u05 coloured by chain, front view.
PDB entry 7u05 coloured by chain, side view.
PDB entry 7u05 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a TRAPPII-Rab11 activation intermediate reveals GTPase substrate selection mechanisms.
Bagde SR, Fromme JC
OpenAccess logo Sci Adv 8 eabn7446 (2022)
PMID: 35559680
Related structures: EMD-26254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279912 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
Trafficking protein particle complex II-specific subunit 130 Chains: M, m
Molecule details ›
Chains: M, m
Length: 242 amino acids
Theoretical weight: 20.61 KDa
Source organism: Saccharomyces cerevisiae
Trafficking protein particle complex II-specific subunit 65 Chains: C, c
Molecule details ›
Chains: C, c
Length: 560 amino acids
Theoretical weight: 63.43 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32893 (Residues: 1-560; Coverage: 100%)
Gene names: KRE11, TRS65, YGR166W
Sequence domains: TRAPP trafficking subunit Trs65 IgD3
TRAPP-associated protein TCA17 Chains: D, d
Trafficking protein particle complex subunit 33 Chains: E, e
Trafficking protein particle complex subunit BET3 Chains: F, I, f, i
Trafficking protein particle complex subunit BET5 Chains: G, g
Trafficking protein particle complex subunit 23 Chains: H, h
Trafficking protein particle complex subunit 31 Chains: J, j
Trafficking protein particle complex subunit 20 Chains: K, k
GTP-binding protein YPT32/YPT11 Chains: L, l
Trafficking protein particle complex II-specific subunit 130 Chains: B, b
Trafficking protein particle complex II-specific subunit 120 Chains: A, a
Trafficking protein particle complex II-specific subunit 65 Chains: N, n

Ligands and Environments

1 bound ligand:
Ligand PLM 4 x PLM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.7Å
Relevant EMDB volumes: EMD-26254
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

The TRAPP complexes: oligomeric exchange factors that activate the small GTPases Rab1 and Rab11.
Galindo et al. (2023)
1 more

1 mention without citation

Structural basis for Rab6 activation by the Ric1-Rgp1 complex.
Feathers et al. (2024)