› 7ubz

X-ray diffraction
1.75Å resolution

Chymotrypsin digested toxin/immunity complex for a T6SS lipase effector from E. cloacae

Released:
DOI: 10.2210/pdb7ubz/pdb
PDB entry 7ubz coloured by chain, front view.
PDB entry 7ubz coloured by chain, side view.
PDB entry 7ubz coloured by chain, top view.
Source organism: Enterobacter cloacae
Entry authors: Cuthbert BJ, Jensen SJ, Goulding CW, Hayes CS

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101842 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ankyrin repeat domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 230 amino acids
Theoretical weight: 25.5 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3CKN4 (Residues: 24-253; Coverage: 91%)
Gene name: ECL_01554
Sequence domains: Ankyrin repeats (3 copies)
Phospholipase Chains: B, D
Molecule details ›
Chains: B, D
Length: 313 amino acids
Theoretical weight: 33.83 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M7ENE2 (Residues: 172-472; Coverage: 64%)
Gene name: NCTC10005_04014

Ligands and Environments

3 bound ligands:
Ligand GOL 9 x GOL
Ligand FLC 1 x FLC
Ligand MIE 2 x MIE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 87.172Å b: 120.493Å c: 122.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.188
Expression system: Escherichia coli

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