› 7ul4

Electron Microscopy
2.8Å resolution

CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6

Released:
DOI: 10.2210/pdb7ul4/pdb
PDB entry 7ul4 coloured by chain, front view.
PDB entry 7ul4 coloured by chain, side view.
PDB entry 7ul4 coloured by chain, top view.
Source organisms:
Primary publication:
Structure determination of inactive-state GPCRs with a universal nanobody.
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022)
PMID: 36396979
Related structures: EMD-26591

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276214 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mu-type opioid receptor Chain: A
Molecule details ›
Chain: A
Length: 426 amino acids
Theoretical weight: 47.92 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42866 (Residues: 6-398; Coverage: 99%)
Gene names: Mor, Oprm, Oprm1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Megabody 6 Chain: D
Molecule details ›
Chain: D
Length: 516 amino acids
Theoretical weight: 55.66 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NG0 1 x NG0
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.8Å
Relevant EMDB volumes: EMD-26591
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

11 review citations

Putting on molecular weight: Enabling cryo-EM structure determination of sub-100-kDa proteins.
Wentinck et al. (2022)
10 more

6 mentions without citation

Three-Dimensional Structural Insights Have Revealed the Distinct Binding Interactions of Agonists, Partial Agonists, and Antagonists with the µ Opioid Receptor.
Li et al. (2023)
5 more