7um7

Electron Microscopy
2.75Å resolution

CryoEM structure of Go-coupled 5-HT5AR in complex with Methylergometrine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225658 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
5-hydroxytryptamine receptor 5A Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47898 (Residues: 32-357; Coverage: 91%)
Gene name: HTR5A
Sequence domains: 7 transmembrane receptor (rhodopsin family)
miniGo protein Chain: B
Molecule details ›
Chain: B
Length: 225 amino acids
Theoretical weight: 25.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Single-chain variable fragment scFv16 Chain: E

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.75Å
Relevant EMDB volumes: EMD-26599
Expression system: Spodoptera frugiperda