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› 7unb

X-ray diffraction
2.18Å resolution

Crystal structure of malaria transmission-blocking antigen Pfs48/45-6C variant in complex with human antibodies RUPA-117 and RUPA-47

Released:
DOI: 10.2210/pdb7unb/pdb
Model geometry
Fit model/data
PDB entry 7unb coloured by chain, front view.
PDB entry 7unb coloured by chain, side view.
PDB entry 7unb coloured by chain, top view.
Source organisms:
Primary publication:
Vaccination with a structure-based stabilized version of malarial antigen Pfs48/45 elicits ultra-potent transmission-blocking antibody responses.
McLeod B, Mabrouk MT, Miura K, Ravichandran R, Kephart S, Hailemariam S, Pham TP, Semesi A, Kucharska I, Kundu P, Huang WC, Johnson M, Blackstone A, Pettie D, Murphy M, Kraft JC, Leaf EM, Jiao Y, van de Vegte-Bolmer M, van Gemert GJ, Ramjith J, King CR, MacGill RS, Wu Y, Lee KK, Jore MM, King NP, Lovell JF, Julien JP
OpenAccess logo Immunity (2022)
PMID: 35977542

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226319 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
RUPA-47 Fab kappa chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Gametocyte surface protein P45/48 Chain: R
Molecule details ›
Chain: R
Length: 147 amino acids
Theoretical weight: 16.52 KDa
Source organism: Plasmodium falciparum
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8I6T1 (Residues: 291-428; Coverage: 33%)
Gene names: PF13_0247, PF3D7_1346700, PF45/48, PFS45-48, PFS45/48
Sequence domains: Sexual stage antigen s48/45 domain
RUPA-117 Fab kappa chain Chain: E
RUPA-47 Fab heavy chain Chain: H
RUPA-117 Fab heavy chain Chain: F

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 73.669Å b: 127.16Å c: 132.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.228
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Host-parasite interactions during Plasmodium infection: Implications for immunotherapies.
Chandley et al. (2022)
4 more

3 mentions without citation

Vaccination with a structure-based stabilized version of malarial antigen Pfs48/45 elicits ultra-potent transmission-blocking antibody responses.
McLeod et al. (2022)
2 more