7uo0

Electron Microscopy
3.4Å resolution

E.coli RNaseP Holoenzyme with Mg2+

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNAprecursor.
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-302258 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Ribonuclease P protein component Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Y8 (Residues: 1-119; Coverage: 100%)
Gene names: JW3681, b3704, rnpA
Sequence domains: Ribonuclease P
RNase P RNA Chain: B
Molecule details ›
Chain: B
Length: 373 nucleotides
Theoretical weight: 121.19 KDa
Source organism: Escherichia coli
Expression system: in vitro transcription vector pT7-TP(deltai)
Sequence domains: Bacterial RNase P class A
Precursor tRNA substrate G(-1) G(-2) Chain: C

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.4Å
Relevant EMDB volumes: EMD-26636
Expression systems:
  • Escherichia coli
  • in vitro transcription vector pT7-TP(deltai)