› 7uo0

Electron Microscopy
3.4Å resolution

E.coli RNaseP Holoenzyme with Mg2+

Released:
DOI: 10.2210/pdb7uo0/pdb
PDB entry 7uo0 coloured by chain, front view.
PDB entry 7uo0 coloured by chain, side view.
PDB entry 7uo0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.
Zhu J, Huang W, Zhao J, Huynh L, Taylor DJ, Harris ME
OpenAccess logo Nat Commun 13 5120 (2022)
PMID: 36045135
Related structures: EMD-26636

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNAprecursor.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-302258 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Ribonuclease P protein component Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Y8 (Residues: 1-119; Coverage: 100%)
Gene names: JW3681, b3704, rnpA
Sequence domains: Ribonuclease P
RNase P RNA Chain: B
Molecule details ›
Chain: B
Length: 373 nucleotides
Theoretical weight: 121.19 KDa
Source organism: Escherichia coli
Expression system: in vitro transcription vector pT7-TP(deltai)
Sequence domains: Bacterial RNase P class A
Precursor tRNA substrate G(-1) G(-2) Chain: C

Ligands and Environments

1 bound ligand:
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.4Å
Relevant EMDB volumes: EMD-26636
Expression systems:
  • Escherichia coli
  • in vitro transcription vector pT7-TP(deltai)

Quick links

Citations

8 review citations

Recognition of the tRNA structure: Everything everywhere but not all at once.
Zhang J. (2024)
7 more

2 mentions without citation

The discovery of a catalytic RNA within RNase P and its legacy.
Kirsebom et al. (2024)
1 more