› 7ury

X-ray diffraction
2.64Å resolution

Tetradecameric hub domain of CaMKII beta

Released:
DOI: 10.2210/pdb7ury/pdb
PDB entry 7ury coloured by chain, front view.
PDB entry 7ury coloured by chain, side view.
PDB entry 7ury coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polymer-based microfluidic device for on-chip counter-diffusive crystallization and in situ X-ray crystallography at room temperature.
Saha S, Özden C, Samkutty A, Russi S, Cohen A, Stratton MM, Perry SL
Lab Chip 23 2075-2090 (2023)
PMID: 36942575

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium/calmodulin-dependent protein kinase type II subunit beta Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 137 amino acids
Theoretical weight: 15.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13554 (Residues: 534-666; Coverage: 20%)
Gene names: CAM2, CAMK2, CAMK2B, CAMKB
Sequence domains: Calcium/calmodulin dependent protein kinase II association domain

Ligands and Environments

1 bound ligand:
Ligand MLT 4 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: C2221
Unit cell:
a: 105.712Å b: 182.795Å c: 110.521Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.
Gu et al. (2023)

1 mention without citation

Polymer-based microfluidic device for on-chip counter-diffusive crystallization and in situ X-ray crystallography at room temperature.
Saha et al. (2023)