› 7us2

Electron Microscopy
2.76Å resolution

PARL-cleaved Skd3 (human ClpB) E455Q Nucleotide Binding Domain hexamer bound to ATPgammaS, open conformation

Released:
DOI: 10.2210/pdb7us2/pdb
PDB entry 7us2 coloured by chain, front view.
PDB entry 7us2 coloured by chain, side view.
PDB entry 7us2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding.
Gupta A, Lentzsch AM, Siegel A, Yu Z, Chio US, Cheng Y, Shan SO
OpenAccess logo Sci Adv 9 eadf5336 (2023)
PMID: 37163603
Related structures: EMD-26722

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214937 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial disaggregase, cleaved form Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 581 amino acids
Theoretical weight: 65.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H078 (Residues: 127-707; Coverage: 82%)
Gene names: CLPB, SKD3
Sequence domains:
Substrate Chain: P
Molecule details ›
Chain: P
Length: 14 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand AGS 6 x AGS
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.76Å
Relevant EMDB volumes: EMD-26722
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding.
Gupta et al. (2023)