› 7uvr

X-ray diffraction
2.86Å resolution

Crystal structure of human ClpP protease in complex with TR-65

Released:
DOI: 10.2210/pdb7uvr/pdb
Model geometry
Fit model/data
PDB entry 7uvr coloured by chain, front view.
PDB entry 7uvr coloured by chain, side view.
PDB entry 7uvr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Potent ClpP agonists with anticancer properties bind with improved structural complementarity and alter the mitochondrial N-terminome.
Mabanglo MF, Wong KS, Barghash MM, Leung E, Chuang SHW, Ardalan A, Majaesic EM, Wong CJ, Zhang S, Lang H, Karanewsky DS, Iwanowicz AA, Graves LM, Iwanowicz EJ, Gingras AC, Houry WA
Structure (2022)
PMID: 36586405

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent Clp protease proteolytic subunit, mitochondrial Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 221 amino acids
Theoretical weight: 24.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16740 (Residues: 58-277; Coverage: 79%)
Gene name: CLPP
Sequence domains: Clp protease

Ligands and Environments

1 bound ligand:
Ligand PJF 7 x PJF
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2
Unit cell:
a: 142.171Å b: 152.515Å c: 104.268Å
α: 90° β: 118.1° γ: 90°
R-values:
R R work R free
0.205 0.203 0.253
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Targeting Mitochondria with ClpP Agonists as a Novel Therapeutic Opportunity in Breast Cancer.
Wedam et al. (2023)
1 more