› 7v1f

X-ray diffraction
2Å resolution

Crystal structure of Omsk hemorrhagic fever virus NS5 MTase (in complex with SAM and GTP)

Released:
DOI: 10.2210/pdb7v1f/pdb
PDB entry 7v1f coloured by chain, front view.
PDB entry 7v1f coloured by chain, side view.
PDB entry 7v1f coloured by chain, top view.
Source organism: Omsk hemorrhagic fever virus
Primary publication:
Crystal Structures of Flavivirus NS5 Guanylyltransferase Reveal a GMP-Arginine Adduct.
Jia H, Zhong Y, Peng C, Gong P
J Virol e0041822 (2022)
PMID: 35758665

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Core protein Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.95 KDa
Source organism: Omsk hemorrhagic fever virus
Expression system: Escherichia coli
UniProt:
  • Canonical: C4TPE0 (Residues: 2518-2775; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand GOL 2 x GOL
Ligand SAM 1 x SAM
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 34.797Å b: 88.059Å c: 42.664Å
α: 90° β: 90.75° γ: 90°
R-values:
R R work R free
0.173 0.172 0.206
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Dengue virus serotypic replacement of NS3 protease or helicase domain causes chimeric viral attenuation but can be recovered by a compensated mutation at helicase domain or NS2B, respectively.
Teramoto T. (2023)
1 more

1 mention without citation

Crystal Structures of Flavivirus NS5 Guanylyltransferase Reveal a GMP-Arginine Adduct.
Jia et al. (2022)