› 7v2z

X-ray diffraction
2.1Å resolution

ZIKV NS3helicase in complex with ssRNA and ATP-Mn2+

Released:
DOI: 10.2210/pdb7v2z/pdb
PDB entry 7v2z coloured by chain, front view.
PDB entry 7v2z coloured by chain, side view.
PDB entry 7v2z coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of Zika Virus Helicase in RNA Unwinding and ATP Hydrolysis.
Lin M, Cui W, Tian H, Zhang Y, Chen C, Yang X, Chi H, Mu Z, Chen C, Wang Z, Ji X, Yang H, Lin Z
ACS Infect Dis 8 150-158 (2022)
PMID: 34904824

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100063 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Serine protease NS3 Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 49.59 KDa
Source organism: Zika virus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A024B7W1 (Residues: 1680-2119; Coverage: 13%)
Sequence domains:
RNA (5'-R(*AP*GP*AP*UP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.57 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 52.62Å b: 72.356Å c: 59.507Å
α: 90° β: 94.154° γ: 90°
R-values:
R R work R free
0.191 0.189 0.239
Expression systems:
  • Escherichia coli
  • Not provided

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