› 7v6d

X-ray diffraction
2.5Å resolution

Structure of lipase B from Lasiodiplodia theobromae

Released:
DOI: 10.2210/pdb7v6d/pdb
PDB entry 7v6d coloured by chain, front view.
PDB entry 7v6d coloured by chain, side view.
PDB entry 7v6d coloured by chain, top view.
Source organism: Lasiodiplodia theobromae
Primary publication:
A Novel Lipase from Lasiodiplodia theobromae Efficiently Hydrolyses C8-C10 Methyl Esters for the Preparation of Medium-Chain Triglycerides' Precursors.
Ng AMJ, Yang R, Zhang H, Xue B, Yew WS, Nguyen GKT
Int J Mol Sci 22 (2021)
PMID: 34638680

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 45.08 KDa
Source organism: Lasiodiplodia theobromae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A5N5DNA6 (Residues: 19-449; Coverage: 100%)
Gene names: DBV05_g2145, LIPB

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I222
Unit cell:
a: 65.428Å b: 162.639Å c: 166.674Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.243
Expression system: Komagataella pastoris

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Citations

2 mentions without citation

A Novel Lipase from Lasiodiplodia theobromae Efficiently Hydrolyses C8-C10 Methyl Esters for the Preparation of Medium-Chain Triglycerides' Precursors.
Ng et al. (2021)
1 more