› 7v94

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the Cas12c2-sgRNA-target DNA ternary complex

Released:
DOI: 10.2210/pdb7v94/pdb
PDB entry 7v94 coloured by chain, front view.
PDB entry 7v94 coloured by chain, side view.
PDB entry 7v94 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the type V-C CRISPR-Cas effector enzyme.
Kurihara N, Nakagawa R, Hirano H, Okazaki S, Tomita A, Kobayashi K, Kusakizako T, Nishizawa T, Yamashita K, Scott DA, Nishimasu H, Nureki O
OpenAccess logo Mol Cell 82 1865-1877.e4 (2022)
PMID: 35366394
Related structures: EMD-31808

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-122205 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Cas12c2 Chain: A
Molecule details ›
Chain: A
Length: 1232 amino acids
Theoretical weight: 138.35 KDa
Source organism: uncultured archaeon
Expression system: Escherichia coli
target DNA (target strand) Chain: C
Molecule details ›
Chain: C
Length: 33 nucleotides
Theoretical weight: 10.08 KDa
Source organism: synthetic construct
Expression system: Not provided
target DNA (non target strand) Chain: D
sgRNA Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.7Å
Relevant EMDB volumes: EMD-31808
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Advances in CRISPR/Cas gene therapy for inborn errors of immunity.
Liu et al. (2023)
2 more

1 mention without citation

Structure of the type V-C CRISPR-Cas effector enzyme.
Kurihara et al. (2022)