› 7vc5

X-ray diffraction
1.61Å resolution

Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in Complex with inhibitor T35 and Febrifugine (FF) at 1.61 A resolution

Released:
DOI: 10.2210/pdb7vc5/pdb
PDB entry 7vc5 coloured by chain, front view.
PDB entry 7vc5 coloured by chain, side view.
PDB entry 7vc5 coloured by chain, top view.
Source organism: Toxoplasma gondii
Entry authors: Manickam Y, Malhotra N, Sharma A

Function and Biology Details

Reaction catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proline--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 57.94 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A7J6JUK2 (Residues: 215-711; Coverage: 70%)
Gene name: TGRH88_057780
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand 1TI 1 x 1TI
Ligand 9SF 1 x 9SF
Ligand MES 1 x MES
Ligand EDO 7 x EDO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 115.194Å b: 73.092Å c: 74.936Å
α: 90° β: 111.57° γ: 90°
R-values:
R R work R free
0.166 0.165 0.196
Expression system: Escherichia coli

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