› 7vgc

X-ray diffraction
2.72Å resolution

Crystal structure of prolyl oligopeptidase from Microbulbifer arenaceous complex with a transition state analog inhibitor ZPR

Released:
DOI: 10.2210/pdb7vgc/pdb
PDB entry 7vgc coloured by chain, front view.
PDB entry 7vgc coloured by chain, side view.
PDB entry 7vgc coloured by chain, top view.
Source organism: Microbulbifer arenaceous
Primary publication:
The structure and molecular dynamics of prolyl oligopeptidase from Microbulbifer arenaceous provide insights into catalytic and regulatory mechanisms.
Huang P, Lv A, Yan Q, Jiang Z, Yang S
Acta Crystallogr D Struct Biol 78 735-751 (2022)
PMID: 35647921

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
prolyl oligopeptidase Chain: A
Molecule details ›
Chain: A
Length: 711 amino acids
Theoretical weight: 79.95 KDa
Source organism: Microbulbifer arenaceous
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

2 bound ligands:
Ligand ZPR 1 x ZPR
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P21
Unit cell:
a: 62.108Å b: 65.622Å c: 91.765Å
α: 90° β: 105.29° γ: 90°
R-values:
R R work R free
0.199 0.195 0.271
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 citation in other articles

Crystal Structure of Inhibitor-Bound Bacterial Oligopeptidase B in the Closed State: Similarity and Difference between Protozoan and Bacterial Enzymes.
Petrenko et al. (2023)
1 more