› 7vl3

X-ray diffraction
1.82Å resolution

The complex structure of beta-1,2-glucosyltransferase from Ignavibacterium album with phenyl alpha-D-glucoside

Released:
DOI: 10.2210/pdb7vl3/pdb
PDB entry 7vl3 coloured by chain, front view.
PDB entry 7vl3 coloured by chain, side view.
PDB entry 7vl3 coloured by chain, top view.
Source organism: Ignavibacterium album JCM 16511
Primary publication:
Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages.
Kobayashi K, Shimizu H, Tanaka N, Kuramochi K, Nakai H, Nakajima M, Taguchi H
J Biol Chem 298 101606 (2022)
PMID: 35065074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase 35 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 711 amino acids
Theoretical weight: 83.17 KDa
Source organism: Ignavibacterium album JCM 16511
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: I0AIT9 (Residues: 6-716; Coverage: 99%)
Gene name: IALB_1185
Sequence domains: Glycosyl hydrolases family 35

Ligands and Environments

2 bound ligands:
Ligand 7OL 2 x 7OL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 165.124Å b: 71.523Å c: 130.151Å
α: 90° β: 105.004° γ: 90°
R-values:
R R work R free
0.164 0.162 0.203
Expression system: Escherichia coli BL21

Quick links

Citations

1 mention without citation

Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages.
Kobayashi et al. (2022)