› 7vo5

X-ray diffraction
2.4Å resolution

Pimaricin type I PKS thioesterase domain (holo Pim TE)

Released:
DOI: 10.2210/pdb7vo5/pdb
PDB entry 7vo5 coloured by chain, front view.
PDB entry 7vo5 coloured by chain, side view.
PDB entry 7vo5 coloured by chain, top view.
Source organism: Streptomyces chattanoogensis
Primary publication:
Structural and Mechanistic Insights into Chain Release of the Polyene PKS Thioesterase Domain
Zhou Y, Tao W, Qi Z, Wei J, Shi T, Kang Q, Zheng J, Zhao Y, Bai L
ACS Catalysis 12 762-776 (2022)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123284 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 30.9 KDa
Source organism: Streptomyces chattanoogensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F1CLA7 (Residues: 1736-2021; Coverage: 14%)
Gene name: scnS4
Sequence domains: Thioesterase domain

Ligands and Environments

1 bound ligand:
Ligand 81E 1 x 81E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P64
Unit cell:
a: 95.449Å b: 95.449Å c: 102.708Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.232
Expression system: Escherichia coli BL21(DE3)

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