› 7voc

X-ray diffraction
2.62Å resolution

The crystal structure of a Radical SAM Enzyme BlsE involved in the Biosynthesis of Blasticidin S

Released:
DOI: 10.2210/pdb7voc/pdb
PDB entry 7voc coloured by chain, front view.
PDB entry 7voc coloured by chain, side view.
PDB entry 7voc coloured by chain, top view.
Source organism: Streptomyces griseochromogenes
Primary publication:
Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical-Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.
Lee YH, Hou X, Chen R, Feng J, Liu X, Ruszczycky MW, Gao JM, Wang B, Zhou J, Liu HW
J Am Chem Soc 144 4478-4486 (2022)
PMID: 35238201

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Radical SAM core domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 364 amino acids
Theoretical weight: 40.33 KDa
Source organism: Streptomyces griseochromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1B1AYF2 (Residues: 1-344; Coverage: 100%)
Gene name: AVL59_19980
Sequence domains: Radical SAM superfamily

Ligands and Environments

4 bound ligands:
Ligand SF4 2 x SF4
Ligand SAM 1 x SAM
Ligand 7QO 1 x 7QO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: I4122
Unit cell:
a: 163.92Å b: 163.92Å c: 158.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.234
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Importance of aspartic acid side chain carboxylate-arginine interaction in substrate selection of arginine 2,3-aminomutase BlsG.
Luo et al. (2023)
1 more

1 mention without citation

Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical-Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.
Lee et al. (2022)