› 7vrg

X-ray diffraction
2.4Å resolution

Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B

Released:
DOI: 10.2210/pdb7vrg/pdb
Model geometry
Fit model/data
PDB entry 7vrg coloured by chain, front view.
PDB entry 7vrg coloured by chain, side view.
PDB entry 7vrg coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
Lynamicin B is a Potential Pesticide by Acting as a Lepidoptera-Exclusive Chitinase Inhibitor.
Lu Q, Xu L, Liu L, Zhou Y, Liu T, Song Y, Ju J, Yang Q
J Agric Food Chem 69 14086-14091 (2021)
PMID: 34797675

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH18 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 540 amino acids
Theoretical weight: 59.99 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: Q4AE59 (Residues: 18-553; Coverage: 100%)
Gene name: OfChi-h
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand 7U8 2 x 7U8
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 70.908Å b: 77.859Å c: 81.972Å
α: 113.634° β: 109.902° γ: 95°
R-values:
R R work R free
0.234 0.234 0.243
Expression system: Komagataella phaffii CBS 7435

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Citations

1 citations in other articles

Chitinase inhibition induces transcriptional dysregulation altering ecdysteroid-mediated control of Spodoptera frugiperda development.
Barbole et al. (2024)