› 7vrs

X-ray diffraction
2.6Å resolution

The complex of Acyltransferase and Acyl Carrier Protein Domains from module 9 of Salinomycin Polyketide Synthase

Released:
DOI: 10.2210/pdb7vrs/pdb
PDB entry 7vrs coloured by chain, front view.
PDB entry 7vrs coloured by chain, side view.
PDB entry 7vrs coloured by chain, top view.
Source organism: Streptomyces albus subsp. albus
Primary publication:
Structural visualization of transient interactions between the cis-acting acyltransferase and acyl carrier protein of the salinomycin modular polyketide synthase.
Feng Y, Zhang F, Huang S, Deng Z, Bai L, Zheng J
Acta Crystallogr D Struct Biol 78 779-791 (2022)
PMID: 35647924

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124802 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polyketide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 48.56 KDa
Source organism: Streptomyces albus subsp. albus
Expression system: Escherichia coli
UniProt:
  • Canonical: H6D573 (Residues: 2583-3027; Coverage: 12%)
Gene names: SLNWT_0276, slnA5
Sequence domains:
Polyketide synthase Chain: C
Molecule details ›
Chain: C
Length: 107 amino acids
Theoretical weight: 11.28 KDa
Source organism: Streptomyces albus subsp. albus
Expression system: Escherichia coli
UniProt:
  • Canonical: H6D573 (Residues: 3585-3670; Coverage: 2%)
Gene names: SLNWT_0276, slnA5
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

2 bound ligands:
Ligand ME9 2 x ME9
Ligand PNS 1 x PNS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P2221
Unit cell:
a: 75.613Å b: 102.061Å c: 150.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.225
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Enzymology of assembly line synthesis by modular polyketide synthases.
Grininger M. (2023)
1 other article