› 7vsk

X-ray diffraction
2.31Å resolution

Crystal structure of E.coli pseudouridine kinase PsuK complexed with pseudouridine.

Released:
DOI: 10.2210/pdb7vsk/pdb
PDB entry 7vsk coloured by chain, front view.
PDB entry 7vsk coloured by chain, side view.
PDB entry 7vsk coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Structure Characterization of Escherichia coli Pseudouridine Kinase PsuK.
Li X, Li K, Guo W, Wen Y, Meng C, Wu B
Front Microbiol 13 926099 (2022)
PMID: 35783380

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 33.78 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N873 (Residues: 1-313; Coverage: 100%)
Gene name: ECBD_1492
Sequence domains: pfkB family carbohydrate kinase

Ligands and Environments

1 bound ligand:
Ligand FJF 1 x FJF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P6322
Unit cell:
a: 182.634Å b: 182.634Å c: 51.228Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.276
Expression system: Escherichia coli BL21(DE3)

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