› 7vvb

X-ray diffraction
1.7Å resolution

Crystal Structure of KRas4A(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1

Released:
DOI: 10.2210/pdb7vvb/pdb
Model geometry
Fit model/data
PDB entry 7vvb coloured by chain, front view.
PDB entry 7vvb coloured by chain, side view.
PDB entry 7vvb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Ras regulation by SIN1.
Zheng Y, Ding L, Meng X, Potter M, Kearney AL, Zhang J, Sun J, James DE, Yang G, Zhou C
OpenAccess logo Proc Natl Acad Sci U S A 119 e2119990119 (2022)
PMID: 35522713

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134022 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Target of rapamycin complex 2 subunit MAPKAP1 Chain: B
Molecule details ›
Chain: B
Length: 522 amino acids
Theoretical weight: 59.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BPZ7 (Residues: 1-522; Coverage: 100%)
Gene names: MAPKAP1, MIP1, SIN1
Sequence domains:
GTPase KRas Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-189; Coverage: 100%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P6122
Unit cell:
a: 101.66Å b: 101.66Å c: 124.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Unmasking the tumourigenic role of SIN1/MAPKAP1 in the mTOR complex 2.
Ezine et al. (2023)
4 other articles

1 mention without citation

3DVizSNP: a tool for rapidly visualizing missense mutations identified in high throughput experiments in iCn3D.
Sierk et al. (2023)