› 7vwn

X-ray diffraction
1.45Å resolution

The structure of an engineered PET hydrolase

Released:
DOI: 10.2210/pdb7vwn/pdb
PDB entry 7vwn coloured by chain, front view.
PDB entry 7vwn coloured by chain, side view.
PDB entry 7vwn coloured by chain, top view.
Source organism: Ideonella sakaiensis
Entry authors: Xie W, Jia Q

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 28.52 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 29-290; Coverage: 100%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 115.588Å b: 50.536Å c: 41.37Å
α: 90° β: 92.458° γ: 90°
R-values:
R R work R free
0.156 0.155 0.179
Expression system: Escherichia coli

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