› 7vy5

Electron Microscopy
3.15Å resolution

Coxsackievirus B3 (VP3-234Q) incubation with CD55 at pH7.4

Released:
DOI: 10.2210/pdb7vy5/pdb
PDB entry 7vy5 coloured by chain, front view.
PDB entry 7vy5 coloured by chain, side view.
PDB entry 7vy5 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains.
Wang Q, Yang Q, Liu C, Wang G, Song H, Shang G, Peng R, Qu X, Liu S, Cui Y, Wang P, Xu W, Zhao X, Qi J, Yang M, Gao GF
Proc Natl Acad Sci U S A 119 (2022)
PMID: 35046043
Related structures: EMD-32194

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136774 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.17 KDa
Source organism: Coxsackievirus B3
Expression system: Homo sapiens
UniProt:
  • Canonical: P03313 (Residues: 583-850; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP2 Chain: B
Molecule details ›
Chain: B
Length: 256 amino acids
Theoretical weight: 28.08 KDa
Source organism: Coxsackievirus B3
Expression system: Homo sapiens
UniProt:
  • Canonical: P03313 (Residues: 77-332; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.23 KDa
Source organism: Coxsackievirus B3
Expression system: Homo sapiens
UniProt:
  • Canonical: P03313 (Residues: 333-570; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 68 amino acids
Theoretical weight: 7.35 KDa
Source organism: Coxsackievirus B3
Expression system: Homo sapiens
UniProt:
  • Canonical: P03313 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Complement decay-accelerating factor Chain: E
Molecule details ›
Chain: E
Length: 123 amino acids
Theoretical weight: 13.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08174 (Residues: 98-220; Coverage: 35%)
Gene names: CD55, CR, DAF
Sequence domains: Sushi repeat (SCR repeat)

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-32194
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18.
Chen et al. (2022)

1 mention without citation

Switching of Receptor Binding Poses between Closely Related Enteroviruses.
Zhou et al. (2022)