› 7vz3

Electron Microscopy
2.46Å resolution

Cryo-EM structure of Depo32, a Klebsiella phage depolymerase targets the K2 serotype K. pneumoniae

Released:
DOI: 10.2210/pdb7vz3/pdb
PDB entry 7vz3 coloured by chain, front view.
PDB entry 7vz3 coloured by chain, side view.
PDB entry 7vz3 coloured by chain, top view.
Source organism: Klebsiella phage GH-K3
Primary publication:
Structural biology and functional features of phage-derived depolymerase Depo32 on Klebsiella pneumoniae with K2 serotype capsular polysaccharides.
Cai R, Ren Z, Zhao R, Lu Y, Wang X, Guo Z, Song J, Xiang W, Du R, Zhang X, Han W, Ru H, Gu J
Microbiol Spectr 11 e0530422 (2023)
PMID: 37750730
Related structures: EMD-32219

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Depolymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 928 amino acids
Theoretical weight: 100.95 KDa
Source organism: Klebsiella phage GH-K3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3S7W7I3 (Residues: 1-907; Coverage: 100%)
Gene name: GHK3_32

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.46Å
Relevant EMDB volumes: EMD-32219
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

A novel phage putative depolymerase, Depo16, has specific activity against K1 capsular-type Klebsiella pneumoniae.
Zhao et al. (2024)