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› 7w1m

Electron Microscopy
6.5Å resolution

Cryo-EM structure of human cohesin-CTCF-DNA complex

Released:
DOI: 10.2210/pdb7w1m/pdb
PDB entry 7w1m coloured by chain, front view.
PDB entry 7w1m coloured by chain, side view.
PDB entry 7w1m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CTCF and R-loops are boundaries of cohesin-mediated DNA looping.
Zhang H, Shi Z, Banigan EJ, Kim Y, Yu H, Bai XC, Finkelstein IJ
Mol Cell 83 2856-2871.e8 (2023)
PMID: 37536339
Related structures: EMD-32252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-216582 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Structural maintenance of chromosomes protein 1A Chain: A
Molecule details ›
Chain: A
Length: 1233 amino acids
Theoretical weight: 143.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14683 (Residues: 1-1233; Coverage: 100%)
Gene names: DXS423E, KIAA0178, SB1.8, SMC1, SMC1A, SMC1L1
Sequence domains:
Structural maintenance of chromosomes protein 3 Chain: B
Molecule details ›
Chain: B
Length: 1217 amino acids
Theoretical weight: 141.77 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UQE7 (Residues: 1-1217; Coverage: 100%)
Gene names: BAM, BMH, CSPG6, SMC3, SMC3L1
Sequence domains:
Double-strand-break repair protein rad21 homolog Chain: C
Cohesin subunit SA-1 Chain: D
Nipped-B-like protein Chain: E
Transcriptional repressor CTCF Chain: H
DNA (118-MER) Chain: F
DNA (118-MER) Chain: G

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand BEF 2 x BEF
Ligand ZN 11 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 6.5Å
Relevant EMDB volumes: EMD-32252
Expression system: Trichoplusia ni

Quick links

Citations

1 citations in other articles

Cohesin and CTCF do not assemble TADs in Xenopus sperm and male pronuclei.
Jessberger et al. (2023)