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› 7w81

X-ray diffraction
1.8Å resolution

Crystal structure of the heme-bound form of the linker-NEAT3 region of IsdH from Staphylococcus aureus

Released:
DOI: 10.2210/pdb7w81/pdb
Model geometry
Fit model/data
PDB entry 7w81 coloured by chain, front view.
PDB entry 7w81 coloured by chain, side view.
PDB entry 7w81 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Structure and role of the linker domain of the iron surface-determinant protein IsdH in heme transportation in Staphylococcus aureus.
Valenciano-Bellido S, Caaveiro JMM, Morante K, Sushko T, Nakakido M, Nagatoishi S, Tsumoto K
OpenAccess logo J Biol Chem 298 101995 (2022)
PMID: 35500652

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-regulated surface determinant protein H Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 21.03 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q931P4 (Residues: 476-656; Coverage: 21%)
Gene names: SAV1731, harA, isdH, sasI
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 49.91Å b: 95.31Å c: 99.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Antibiotic Susceptibility Profiling of Human Pathogenic Staphylococcus aureus Strains Using Whole Genome Sequencing and Genome-Scale Annotation Approaches.
Snoussi et al. (2023)
1 more

2 mentions without citation

Structure and role of the linker domain of the iron surface-determinant protein IsdH in heme transportation in Staphylococcus aureus.
Valenciano-Bellido et al. (2022)
1 more