› 7w8n

X-ray diffraction
1.75Å resolution

Microbial Hormone-sensitive lipase E53 wild type

Released:
DOI: 10.2210/pdb7w8n/pdb
PDB entry 7w8n coloured by chain, front view.
PDB entry 7w8n coloured by chain, side view.
PDB entry 7w8n coloured by chain, top view.
Source organism: Erythrobacter longus
Primary publication:
Mechanism and Structural Insights Into a Novel Esterase, E53, Isolated From Erythrobacter longus.
Ding Y, Nie L, Yang XC, Li Y, Huo YY, Li Z, Gao Y, Cui HL, Li J, Xu XW
Front Microbiol 12 798194 (2021)
PMID: 35069500

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100434 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 314 amino acids
Theoretical weight: 33.16 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A074MDU6 (Residues: 1-314; Coverage: 100%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold
Alpha/beta hydrolase fold-3 domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 314 amino acids
Theoretical weight: 33.1 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A074MDU6 (Residues: 1-314; Coverage: 100%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

11 bound ligands:
Ligand GOL 9 x GOL
Ligand EDO 25 x EDO
Ligand 6NA 5 x 6NA
Ligand DMS 13 x DMS
Ligand CCN 24 x CCN
Ligand D8F 4 x D8F
Ligand SO4 2 x SO4
Ligand DIO 1 x DIO
Ligand BUA 1 x BUA
Ligand NPO 3 x NPO
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Unit cell:
a: 70.576Å b: 129.838Å c: 221.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.179
Expression system: Escherichia coli K-12

Quick links

Citations

2 mentions without citation

Mechanism and Structural Insights Into a Novel Esterase, E53, Isolated From Erythrobacter longus.
Ding et al. (2021)
1 more