› 7wab

X-ray diffraction
1.75Å resolution

Crystal structure of the prolyl endoprotease, PEP, from Aspergillus niger

Released:
DOI: 10.2210/pdb7wab/pdb
PDB entry 7wab coloured by chain, front view.
PDB entry 7wab coloured by chain, side view.
PDB entry 7wab coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Crystal structure and substrate recognition mechanism of the prolyl endoprotease PEP from Aspergillus niger.
Miyazono KI, Kubota K, Takahashi K, Tanokura M
Biochem Biophys Res Commun 591 76-81 (2022)
PMID: 34999257

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
COMPASS (Complex proteins associated with Set1p) component shg1 family protein Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 54.44 KDa
Source organism: Aspergillus niger
UniProt:
  • Canonical: A0A3F3RXI7 (Residues: 43-526; Coverage: 96%)
Gene name: CAN33_0042530
Sequence domains: Serine carboxypeptidase S28

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P4212
Unit cell:
a: 144.517Å b: 144.517Å c: 56.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.193

Quick links

Citations

2 mentions without citation

Post-Proline Cleaving Enzymes (PPCEs): Classification, Structure, Molecular Properties, and Applications.
Baharin et al. (2022)
1 more