› 7wgd

X-ray diffraction
3.2Å resolution

X-ray structure of thermostabilized Drosophila dopamine transporter with GABA transporter1-like substitutions in the binding site, in substrate-free form.

Released:
DOI: 10.2210/pdb7wgd/pdb
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PDB entry 7wgd coloured by chain, front view.
PDB entry 7wgd coloured by chain, side view.
PDB entry 7wgd coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into GABA transport inhibition using an engineered neurotransmitter transporter.
Joseph D, Nayak SR, Penmatsa A
EMBO J e110735 (2022)
PMID: 35796008

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226294 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 60.14 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 25-601; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
Antibody fragment (9D5) Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Antibody fragment (9D5) heavy chain Chain: H

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand Y01 1 x Y01
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 96.388Å b: 140.709Å c: 168.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.286
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

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Citations

2 review citations

A comparative review on the well-studied GAT1 and the understudied BGT-1 in the brain.
Bhatt et al. (2023)
1 more

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