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› 7wt2

X-ray diffraction
2Å resolution

human glyoxalase I in complex with TLSC702

Released:
DOI: 10.2210/pdb7wt2/pdb
Model geometry
Fit model/data
PDB entry 7wt2 coloured by chain, front view.
PDB entry 7wt2 coloured by chain, side view.
PDB entry 7wt2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human glyoxalase I and its complex with TLSC702 reveal inhibitor binding mode and substrate preference.
Usami M, Ando K, Shibuya A, Takasawa R, Yokoyama H
FEBS Lett 596 1458-1467 (2022)
PMID: 35363883

Function and Biology Details

Reaction catalysed: 
(R)-S-lactoylglutathione = methylglyoxal + glutathione.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoylglutathione lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04760 (Residues: 1-184; Coverage: 100%)
Gene name: GLO1
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand 5ZO 2 x 5ZO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 53.691Å b: 82.532Å c: 45.281Å
α: 90° β: 93.85° γ: 90°
R-values:
R R work R free
0.187 0.183 0.228
Expression system: Escherichia coli

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Citations

1 citations in other articles

Structural and mutational studies suggest key residues to determine whether stomatin SPFH domains form dimers or trimers.
Komatsu et al. (2022)