7wtp

Electron Microscopy
3.8Å resolution

Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (with Rps2)

Released:
Source organism: Saccharomyces cerevisiae
Related structures: EMD-32794

Function and Biology Details

Reaction catalysed:
guanosine(1575) in yeast 18S rRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(1575) in yeast 18S rRNA + S-adenosyl-L-homocysteine.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
PDBe Complex ID:
PDB-CPX-301183 (preferred)
Entry contents:
18 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (19 distinct):
Small ribosomal subunit protein eS1A Chain: SB
Molecule details ›
Chain: SB
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33442 (Residues: 1-255; Coverage: 100%)
Gene names: L9753.9, PLC1, RPS10A, RPS1A, YLR441C
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS5 Chain: SC
Molecule details ›
Chain: SC
Length: 254 amino acids
Theoretical weight: 27.49 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25443 (Residues: 1-254; Coverage: 100%)
Gene names: G2893, RPS2, RPS4, SUP38, SUP44, YGL123W
Sequence domains:
Small ribosomal subunit protein eS4A Chain: SE
Small ribosomal subunit protein eS6A Chain: SG
Small ribosomal subunit protein eS7A Chain: SH
Small ribosomal subunit protein eS8A Chain: SI
Small ribosomal subunit protein uS4A Chain: SJ
Small ribosomal subunit protein uS17A Chain: SL
Small ribosomal subunit protein uS15 Chain: SN
Small ribosomal subunit protein uS11A Chain: SO
Small ribosomal subunit protein uS8A Chain: SW
Small ribosomal subunit protein uS12A Chain: SX
Small ribosomal subunit protein eS24A Chain: SY
Small ribosomal subunit protein eS27A Chain: Sb
Small ribosomal subunit protein eS30A Chain: Se
Pre-rRNA-processing protein PNO1 Chain: CA
18S rRNA (guanine(1575)-N(7))-methyltransferase Chain: CB
Ribosome biogenesis protein TSR1 Chain: CC
18S Ribosomal RNA Chain: C2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-32794