› 7wwh

X-ray diffraction
1.92Å resolution

Crystal structure of the Geobacillus thermoglucosidasius feruloyl esterase GthFAE

Released:
DOI: 10.2210/pdb7wwh/pdb
PDB entry 7wwh coloured by chain, front view.
PDB entry 7wwh coloured by chain, side view.
PDB entry 7wwh coloured by chain, top view.
Source organism: Parageobacillus thermoglucosidasius
Primary publication:
Structure-guided rational design of the Geobacillus thermoglucosidasius feruloyl esterase GthFAE to improve its thermostability.
Yang W, Sun L, Dong P, Chen Y, Zhang H, Huang X, Wu L, Chen L, Jing D, Wu Y
Biochem Biophys Res Commun 600 117-122 (2022)
PMID: 35219099

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.18 KDa
Source organism: Parageobacillus thermoglucosidasius
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P21
Unit cell:
a: 46.038Å b: 87.41Å c: 69.456Å
α: 90° β: 95.97° γ: 90°
R-values:
R R work R free
0.176 0.174 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Characterization of feruloyl esterases from Pecoramyces sp. F1 and the synergistic effect in biomass degradation.
Ma et al. (2022)
1 more

1 mention without citation

An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate.
Perez-Garcia et al. (2023)