› 7x0n

X-ray diffraction
1.68Å resolution

Crystal structure of sugar binding protein CbpB complexed wtih laminaribiose from Clostridium thermocellum

Released:
DOI: 10.2210/pdb7x0n/pdb
PDB entry 7x0n coloured by chain, front view.
PDB entry 7x0n coloured by chain, side view.
PDB entry 7x0n coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.
Yan F, Dong S, Liu YJ, Yao X, Chen C, Xiao Y, Bayer EA, Shoham Y, You C, Cui Q, Feng Y
mBio 13 e0147622 (2022)
PMID: 36069444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Extracellular solute-binding protein family 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 45.25 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DE73 (Residues: 50-459; Coverage: 93%)
Gene name: Cthe_1020
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 72.75Å b: 46.44Å c: 116.49Å
α: 90° β: 106.58° γ: 90°
R-values:
R R work R free
0.174 0.173 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Comparative genomics reveals cellobiose hydrolysis mechanism of Ruminiclostridium thermocellum M3, a cellulosic saccharification bacterium.
Tao et al. (2022)
1 more