› 7x1n

X-ray diffraction
3.32Å resolution

Crystal structure of MEF2D-MRE complex

Released:
DOI: 10.2210/pdb7x1n/pdb
PDB entry 7x1n coloured by chain, front view.
PDB entry 7x1n coloured by chain, side view.
PDB entry 7x1n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional, structural and molecular characterizations of leukemogenic driver MEF2D-HNRNPUL1 fusion.
Zhang M, Zhang H, Li Z, Bai L, Wang Q, Li J, Jiang M, Xue Q, Cheng N, Zhang W, Mao D, Chen Z, Huang J, Meng G, Chen Z, Chen SJ
Blood (2022)
PMID: 35544603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122266 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Myocyte-specific enhancer factor 2D Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 98 amino acids
Theoretical weight: 11.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14814 (Residues: 2-95; Coverage: 18%)
Gene name: MEF2D
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
DNA (5'-D(P*AP*AP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*G)-3') Chains: E, K
Molecule details ›
Chains: E, K
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*T)-3') Chains: F, L
Molecule details ›
Chains: F, L
Length: 15 nucleotides
Theoretical weight: 4.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 74.863Å b: 75.651Å c: 111.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.275
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

A typical bedside-to-bench investigation of leukemogenic driver MEF2D fusion reveals new targeted therapy in B-cell acute lymphoblastic leukemia.
Zhang et al. (2022)
1 more