› 7xbn

X-ray diffraction
2Å resolution

Crystal Structure of YC-17-bound cytochrome P450 PikC with the unnatural amino acid p-Acetyl-L-Phenylalanine incorporated at position 238

Released:
DOI: 10.2210/pdb7xbn/pdb
Model geometry
Fit model/data
PDB entry 7xbn coloured by chain, front view.
PDB entry 7xbn coloured by chain, side view.
PDB entry 7xbn coloured by chain, top view.
Source organism: Streptomyces venezuelae
Primary publication:
New mechanistic insight of cytochrome P450 PikC gained from site-specific mutagenesis by non-coding amino acids
Pan YJ, Li GB, Gao X, Li SY
Nat Commun (2023)

Function and Biology Details

Reaction catalysed: 
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase PikC Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 49.39 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli
UniProt:
  • Canonical: O87605 (Residues: 1-416; Coverage: 100%)
Gene names: picK, pikC
Sequence domains: Cytochrome P450

Ligands and Environments

4 bound ligands:
Ligand HEM 2 x HEM
Ligand PXI 2 x PXI
Ligand GOL 4 x GOL
Ligand PEG 3 x PEG
1 modified residue:
Ligand 4AF 2 x 4AF

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 59.823Å b: 108.903Å c: 152.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli

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