› 7xey

X-ray diffraction
2.29Å resolution

EDS1-PAD4 complexed with pRib-ADP

Released:
DOI: 10.2210/pdb7xey/pdb
PDB entry 7xey coloured by chain, front view.
PDB entry 7xey coloured by chain, side view.
PDB entry 7xey coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity.
Huang S, Jia A, Song W, Hessler G, Meng Y, Sun Y, Xu L, Laessle H, Jirschitzka J, Ma S, Xiao Y, Yu D, Hou J, Liu R, Sun H, Liu X, Han Z, Chang J, Parker JE, Chai J
Science 377 eabq3297 (2022)
PMID: 35857645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193415 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein EDS1 Chain: A
Molecule details ›
Chain: A
Length: 623 amino acids
Theoretical weight: 71.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9SU72 (Residues: 1-623; Coverage: 100%)
Gene names: At3g48090, EDS1, EDS1-90, EDS1A, T17F15.40
Sequence domains:
Lipase-like PAD4 Chain: B
Molecule details ›
Chain: B
Length: 541 amino acids
Theoretical weight: 61.05 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9S745 (Residues: 1-541; Coverage: 100%)
Gene names: At3g52430, EDS9, F22O6.190, PAD4
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand RP5 1 x RP5
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 82.357Å b: 91.863Å c: 94.271Å
α: 90° β: 111.79° γ: 90°
R-values:
R R work R free
0.186 0.184 0.232
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

Dying in self-defence: a comparative overview of immunogenic cell death signalling in animals and plants.
Maekawa et al. (2023)
10 more

1 mention without citation

A splicing variant of EDS1 from Vitis vinifera forms homodimers but no heterodimers with PAD4.
Voss et al. (2023)