› 7xgb

X-ray diffraction
2.7Å resolution

Crystal structure of the ctcP from Streptomyces aureofaciens

Released:
DOI: 10.2210/pdb7xgb/pdb
PDB entry 7xgb coloured by chain, front view.
PDB entry 7xgb coloured by chain, side view.
PDB entry 7xgb coloured by chain, top view.
Source organism: Kitasatospora aureofaciens
Primary publication:
Crystal structure determination of the halogenase CtcP from Streptomyces aureofaciens.
Yin L
Acta Crystallogr F Struct Biol Commun 78 270-275 (2022)
PMID: 35787554

Function and Biology Details

Reaction catalysed: 
Tetracycline + FADH(2) + chloride + O(2) + H(+) = 7-chlorotetracycline + FAD + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline 7-halogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 556 amino acids
Theoretical weight: 63.55 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1E7MYN1 (Residues: 1-555; Coverage: 100%)
Gene names: B6264_18525, HS99_0013305, ctcP, cts4
Sequence domains: FAD binding domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Unit cell:
a: 116.393Å b: 121.281Å c: 179.194Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.224
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Site-selective chlorination of pyrrolic heterocycles by flavin dependent enzyme PrnC.
Peh et al. (2024)

1 mention without citation

Crystal structure determination of the halogenase CtcP from Streptomyces aureofaciens.
Yin L. (2022)