› 7xjp

Electron Microscopy
2.71Å resolution

Cryo-EM structure of EDS1 and SAG101 with ATP-APDR

Released:
DOI: 10.2210/pdb7xjp/pdb
PDB entry 7xjp coloured by chain, front view.
PDB entry 7xjp coloured by chain, side view.
PDB entry 7xjp coloured by chain, top view.
Source organism: Arabidopsis
Primary publication:
TIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity.
Jia A, Huang S, Song W, Wang J, Meng Y, Sun Y, Xu L, Laessle H, Jirschitzka J, Hou J, Zhang T, Yu W, Hessler G, Li E, Ma S, Yu D, Gebauer J, Baumann U, Liu X, Han Z, Chang J, Parker JE, Chai J
Science 377 eabq8180 (2022)
PMID: 35857644
Related structures: EMD-33233

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175503 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein EDS1 Chain: A
Molecule details ›
Chain: A
Length: 623 amino acids
Theoretical weight: 71.78 KDa
Source organism: Arabidopsis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9SU72 (Residues: 1-623; Coverage: 100%)
Gene names: At3g48090, EDS1, EDS1-90, EDS1A, T17F15.40
Sequence domains:
Senescence-associated carboxylesterase 101 Chain: B
Molecule details ›
Chain: B
Length: 537 amino acids
Theoretical weight: 62.15 KDa
Source organism: Arabidopsis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q4F883 (Residues: 1-537; Coverage: 100%)
Gene names: At5g14930, F2G14.50, SAG101
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand IPA 1 x IPA
Ligand APR 1 x APR
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.71Å
Relevant EMDB volumes: EMD-33233
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

An Overview of PRR- and NLR-Mediated Immunities: Conserved Signaling Components across the Plant Kingdom That Communicate Both Pathways.
Ramírez-Zavaleta et al. (2022)
11 more