› 7xsj

X-ray diffraction
3.2Å resolution

The structure of the Mint1/Munc18-1/syntaxin-1 complex

Released:
DOI: 10.2210/pdb7xsj/pdb
Model geometry
Fit model/data
PDB entry 7xsj coloured by chain, front view.
PDB entry 7xsj coloured by chain, side view.
PDB entry 7xsj coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A non-canonical target-binding site in Munc18-1 domain 3b for assembling the Mint1-Munc18-1-syntaxin-1 complex.
Li W, Xing Y, Wang Y, Xu T, Song E, Feng W
Structure 31 68-77.e5 (2023)
PMID: 36608665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-128680 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Syntaxin-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 600 amino acids
Theoretical weight: 68.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P61765 (Residues: 1-594; Coverage: 100%)
Gene names: Stxbp1, Unc18a
Sequence domains: Sec1 family
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 249 amino acids
Theoretical weight: 28.88 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P32851 (Residues: 25-267; Coverage: 84%)
Gene names: Sap, Stx1a
Sequence domains:
Amyloid-beta A4 precursor protein-binding family A member 1 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P4212
Unit cell:
a: 156.238Å b: 156.238Å c: 78.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.27
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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