› 7xt3

X-ray diffraction
2.15Å resolution

Crystal Structure of Hepatitis virus A 2C protein 128-335 aa

Released:
DOI: 10.2210/pdb7xt3/pdb
Model geometry
Fit model/data
PDB entry 7xt3 coloured by chain, front view.
PDB entry 7xt3 coloured by chain, side view.
PDB entry 7xt3 coloured by chain, top view.
Source organism: Hepatovirus A
Primary publication:
Biochemical and structural characterization of hepatitis A virus 2C reveals an unusual ribonuclease activity on single-stranded RNA.
Chen P, Wojdyla JA, Colasanti O, Li Z, Qin B, Wang M, Lohmann V, Cui S
OpenAccess logo Nucleic Acids Res (2022)
PMID: 35947700

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein 2C Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.51 KDa
Source organism: Hepatovirus A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08617 (Residues: 1215-1422; Coverage: 9%)
Sequence domains: RNA helicase

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 50.165Å b: 54.687Å c: 85.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Enteroviral 2C protein is an RNA-stimulated ATPase and uses a two-step mechanism for binding to RNA and ATP.
Yeager et al. (2022)
2 more

2 mentions without citation

Picornavirus 2C proteins: structure-function relationships and interactions with host factors.
Yin et al. (2024)
1 more