› 7xts

X-ray diffraction
2.21Å resolution

The apo structure of the engineered TfCut S130A

Released:
DOI: 10.2210/pdb7xts/pdb
PDB entry 7xts coloured by chain, front view.
PDB entry 7xts coloured by chain, side view.
PDB entry 7xts coloured by chain, top view.
Source organism: Thermobifida fusca
Primary publication:
Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases.
Yang Y, Min J, Xue T, Jiang P, Liu X, Peng R, Huang JW, Qu Y, Li X, Ma N, Tsai FC, Dai L, Zhang Q, Liu Y, Chen CC, Guo RT
Nat Commun 14 1645 (2023)
PMID: 36964144

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169124 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha/beta hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 28.22 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER METALJET
Spacegroup: P21
Unit cell:
a: 66.025Å b: 42.959Å c: 80.377Å
α: 90° β: 92.35° γ: 90°
R-values:
R R work R free
0.184 0.179 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes.
Sui et al. (2023)
1 more