› 7xtu

X-ray diffraction
2.43Å resolution

The structure of TfCut S130A

Released:
DOI: 10.2210/pdb7xtu/pdb
PDB entry 7xtu coloured by chain, front view.
PDB entry 7xtu coloured by chain, side view.
PDB entry 7xtu coloured by chain, top view.
Source organism: Thermobifida fusca
Primary publication:
Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases.
Yang Y, Min J, Xue T, Jiang P, Liu X, Peng R, Huang JW, Qu Y, Li X, Ma N, Tsai FC, Dai L, Zhang Q, Liu Y, Chen CC, Guo RT
Nat Commun 14 1645 (2023)
PMID: 36964144

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha/beta hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 261 amino acids
Theoretical weight: 28.3 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

5 bound ligands:
Ligand SO4 2 x SO4
Ligand ACT 9 x ACT
Ligand PEG 6 x PEG
Ligand GOL 1 x GOL
Ligand LI 2 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER METALJET
Spacegroup: P3121
Unit cell:
a: 91.414Å b: 91.414Å c: 247.696Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes.
Sui et al. (2023)
1 more