7xv3

Electron Microscopy
2.76Å resolution

Cryo-EM structure of LPS-bound GPR174 in complex with Gs protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225737 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Probable G-protein coupled receptor 174 Chain: R
Molecule details ›
Chain: R
Length: 333 amino acids
Theoretical weight: 38.54 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXC1 (Residues: 1-333; Coverage: 100%)
Gene names: FKSG79, GPCR17, GPR174
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Engineered G protein subunit S (mini-Gs) Chain: A
Nb35 Chain: N
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.76Å
Relevant EMDB volumes: EMD-33479
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli