› 7y5u

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the monomeric human CAF1LC-H3-H4 complex

Released:
DOI: 10.2210/pdb7y5u/pdb
PDB entry 7y5u coloured by chain, front view.
PDB entry 7y5u coloured by chain, side view.
PDB entry 7y5u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1.
Liu CP, Yu Z, Xiong J, Hu J, Song A, Ding D, Yu C, Yang N, Wang M, Yu J, Hou P, Zeng K, Li Z, Zhang Z, Zhang X, Li W, Zhang Z, Zhu B, Li G, Xu RM
Science 381 eadd8673 (2023)
PMID: 37616371
Related structures: EMD-33625

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226788 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Chromatin assembly factor 1 subunit A Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 47.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13111 (Residues: 442-853; Coverage: 43%)
Gene names: CAF, CAF1P150, CHAF1A
Sequence domains:
Histone H3.1 Chain: D
Molecule details ›
Chain: D
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68431 (Residues: 1-136; Coverage: 100%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chain: E
Histone-binding protein RBBP4 Chain: C
Chromatin assembly factor 1 subunit B Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-33625
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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