› 7y6k

Electron Microscopy
3.34Å resolution

Cryo-EM structure of SARS-CoV-2 receptor binding domain in complex with K202.B bispecific antibody

Released:
DOI: 10.2210/pdb7y6k/pdb
PDB entry 7y6k coloured by chain, front view.
PDB entry 7y6k coloured by chain, side view.
PDB entry 7y6k coloured by chain, top view.
Source organisms:
Primary publication:
Novel bispecific human antibody platform specifically targeting a fully open spike conformation potently neutralizes multiple SARS-CoV-2 variants.
Kim JW, Heo K, Kim HJ, Yoo Y, Cho HS, Jang HJ, Lee HY, Ko IY, Woo JR, Cho YB, Lee JH, Yang HR, Shin HG, Choi HL, Hwang K, Kim S, Kim H, Chun K, Lee S
OpenAccess logo Antiviral Res 212 105576 (2023)
PMID: 36870394
Related structures: EMD-33642

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-291281 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Spike glycoprotein Chain: B
Molecule details ›
Chain: B
Length: 1208 amino acids
Theoretical weight: 133.78 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
scFv region of light chain from K202.B, bispecific antibody Chain: E
Molecule details ›
Chain: E
Length: 246 amino acids
Theoretical weight: 25.6 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab region of light chain from K202.B, bispecific antibody Chain: I
Fab region of heavy chain from K202.B, bispecific antibody Chain: J

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.34Å
Relevant EMDB volumes: EMD-33642
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

1 review citation

Function and mechanism of bispecific antibodies targeting SARS-CoV-2.
Li et al. (2024)
4 other articles