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› 7ye1

Electron Microscopy
3.7Å resolution

The cryo-EM structure of C. crescentus GcrA-TACup

Released:
DOI: 10.2210/pdb7ye1/pdb
PDB entry 7ye1 coloured by chain, front view.
PDB entry 7ye1 coloured by chain, side view.
PDB entry 7ye1 coloured by chain, top view.
Source organism: Caulobacter vibrioides NA1000
Primary publication:
Cryo-EM structures of Caulobacter crescentus transcription activation complex with an essential cell cycle regulator GcrA
Wu XX, Zhang Y
Nucleic Acids Res. (2023)
Related structures: EMD-33761

Function and Biology Details

Reaction catalysed: 
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-101812 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 37.32 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: B8H4F8 (Residues: 1-338; Coverage: 100%)
Gene names: CCNA_01330, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1356 amino acids
Theoretical weight: 151.09 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: B8GZW7 (Residues: 1-1356; Coverage: 100%)
Gene names: CCNA_00536, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
DNA-directed RNA polymerase subunit omega Chain: E
RNA polymerase sigma factor RpoD Chain: F
Cell cycle regulatory protein GcrA Chain: G
DNA (57-MER)-non template Chain: H
DNA (57-MER)-template Chain: I

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 3 x ZN
1 modified residue:
Ligand 6MA 2 x 6MA

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.7Å
Relevant EMDB volumes: EMD-33761
Expression system: Escherichia coli DH5[alpha]

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