7yhs

Electron Microscopy
3.37Å resolution

Structure of Csy-AcrIF4-dsDNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-104781 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
Type I-F CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.31 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3A8DDU9 (Residues: 1-434; Coverage: 100%)
Gene name: ALP65_00954
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy3 Chains: C, D, F, G, H, I
Molecule details ›
Chains: C, D, F, G, H, I
Length: 342 amino acids
Theoretical weight: 37.62 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
CRISPR type I-F/YPEST-associated protein Csy2 Chain: E
AcrIF4 Chain: J
Csy4 Chain: L
DNA (39-MER) Chain: N
DNA (5'-D(P*AP*GP*CP*AP*GP*CP*TP*GP*CP*AP*CP*C)-3') Chain: O
RNA (58-MER) Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.37Å
Relevant EMDB volumes: EMD-33837
Expression system: Escherichia coli